Anti-SIRT1 Antibody

• Catalog Number: orb382038
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SIRT1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IP, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide of human SIRT1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-200, ChIP: 1:20-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SIRT1
• Entrez: 23411
• UniProt ID: Q96EB6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: SIR2L1; NAD-dependent protein deacetylase sirtuin-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SIRT1 expression in Jurkat (A), MCF7 (B) whole cell lysates. (Predicted band size: 61; 81 kD; Observed band size: 120 kD)

Immunofluorescent analysis of SIRT1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.