Sirt1 Antibody (C-term)

SKU: orb306144

Description

Rabbit polyclonal antibody to Sirt1

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD72187
• Immunogen: This Sirt1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 566-601 amino acids from the C-terminal region of human Sirt1.
• Target: Sirt1
• Molecular Weight: 80372
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NAD-dependent protein deacetylase sirtuin-1 antibody, anti 351- antibody, anti Regulatory protein SIR2 homolog 1 antibody, anti SIR2-like protein 1 antibody, anti SIR2alpha antibody, anti Sir2 antibody, anti mSIR2a antibody, anti SirtT1 75 kDa fragment antibody, anti 75SirT1 antibody, anti Sirt1 antibody, anti Sir2l1 antibody

Western blot analysis of lysate from mouse F9 cell line, using Mouse Sirt1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-Sirt1 Antibody (C-term) at 1:2000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of A549 cells stained with Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

Sirt1

UniProt

Q96EB6

D'Onofrio, Nunzia et al. Synergistic Effect of Dietary Betaines on SIRT1-Mediated Apoptosis in Human Oral Squamous Cell Carcinoma Cal 27 Cancers (Basel), 12, E2468 (2020)

PubMed: 32878301