SH3PXD2B Rabbit Polyclonal Antibody

SKU: orb1879179

Description

The SH3PXD2B Antibody is suitable for FC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SH3PXD2B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB (1/500 - 1/1000), FC (1/10 - 1/30)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SH3PXD2B. The exact sequence is proprietary.
• Target: SH3PXD2B
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAD49; KIAA1295; TKS4; SH3 and PX domain-containing protein 2B; Adapter protein HOFI; Factor for adipocyte differentiation 49; Tyrosine kinase substrate with four SH3 domains

Western blot analysis of SH3PXD2B expression in U251MG (A), U87MG (B), SHSY5Y (C) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 120 kD)

Flow cytometric analysis of U2OS cells using Anti-SH3PXD2B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

Gene Symbol

SH3PXD2B

UniProt

A1X283

NCBI Gene ID

285590