SH3PXD2B Antibody (C-Term)

SKU: orb1925797

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD77536
• Immunogen: This SH3PXD2B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from human SH3PXD2B.
• Target: SH3PXD2B
• Molecular Weight: 101579 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SH3 and PX domain-containing protein 2B, Adapter protein HOFI, Factor for adipocyte differentiation 49, Tyrosine kinase substrate with four SH3 domains, SH3PXD2B, FAD49, KIAA1295, TKS4

All lanes: Anti-SH3PXD2B Antibody (C-Term) at 1:2000 dilution. Lane 1: U-251 MG whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing U-2OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

SH3PXD2B

UniProt

A1X283