Anti-SERCA1 Antibody

• Catalog Number: orb213591
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP2A1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SERCA1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATP2A1
• Entrez: 116601, 11937, 487
• UniProt ID: Q8R429, Q64578, O14983
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Sarcoplasmic/endoplasmic reticulum calcium AT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SERCA1 expression in EC9706 (A), DLD (B), CT26 (C) whole cell lysates. (Predicted band size: 110 kD; Observed band size: 110 kD)

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SERCA1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).