AT2A1/SERCA1 ATPase Rabbit Polyclonal Antibody

• Catalog Number: orb101740
• Category: Antibodies
• Description: AT2A1/SERCA1 ATPase Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SERCA1 ATPase (101-200/1001aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110 kDa
• Target: ATP2A1
• UniProt ID: O14983
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: fast twitch skeletal muscle isoform; AT2A1_HUMAN;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AT2A1) Polyclonal Antibody, Unconjugated (orb101740) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (orb101740) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (orb101740) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AT2A1 Polyclonal Antibody, Unconjugated (orb101740) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.