SCIMP Antibody

• Catalog Number: orb154406
• Category: Antibodies
• Description: Mouse monoclonal antibody to SCIMP which also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse.
• Target: SCIMP
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Recombinant intracellular part of human SCIMP
• UniProt ID: Q6UWF3
• Tested applications: FC, ICC, IP, WB
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: NVL-07
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NVL
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 388325

Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

Western blotting analysis of human SCIMP using mouse monoclonal antibody NVL-07 on lysates of human SCIMP transfectants under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SCIMP was detected around 17-22 kDa.

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 µg/ml, GAM APC).

Separation of HeLa cells stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 5 µg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Separation of human monocytes (red-filled) from SCIMP negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 µg/ml, GAM APC).