SCIMP Antibody (PE)

• Catalog Number: orb1570903
• Category: Antibodies
• Description: SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.
• Target: SCIMP
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Recombinant intracellular part of human SCIMP
• UniProt ID: Q6UWF3
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: NVL-07
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: NVL
• Note: For research use only
• Entrez: 388325

Flow cytometry multicolor staining pattern of human lymphocytes using anti-human HLA-DR/DP (MEM-136) APC antibody (10 μl reagent / 100 μl of peripheral whole blood, surface staining) and anti-SCIMP (NVL-07) PE antibody (10 μl reagent / 100 μl of peripheral whole blood, intracellular staining).

Separation of human HLA-DR/DP positive SCIMP positive lymphocytes (red-filled) from HLA-DR/DP negative SCIMP negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-SCIMP (NVL-07) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-SCIMP (NVL-07) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).