Anti-SARS-CoV-2 RBD antibody(DM54); Rabbit mAb

• Catalog Number: orb757388
• Category: Antibodies
• Description: Anti-SARS-CoV-2 RBD antibody(DM54), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM54
• Tested applications: ELISA, FC
• Reactivity: Virus
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S protein RBD
• UniProt ID: P0DTC2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 RBD
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Elisa plate pre-coated by 2 μg/ml (100 μL/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody (clone:DM54) in a linear range of 0.1-100 ng/ml.

Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody (clone: DM54) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1 μg/ml, orb689474) binding to Expi 293 cell line transfected with human ACE2. IC50=816.4 ng/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.