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Anti-S100-B Antibody

Catalog Number: orb411621

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb411621
CategoryAntibodies
DescriptionRabbit polyclonal antibody to S100B
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human S100-B
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetS100B
Entrez25742, 20203, 6285
UniProt IDP50114, P04271, P04631
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-Protein S100-B antibody, anti-S-100 protein b
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-S100-B Antibody

Western blot analysis of S100-B expression in A375 (A) whole cell lysates. (Predicted band size: 10 kD; Observed band size: 11 kD)

Anti-S100-B Antibody

Immunohistochemical analysis of S100-B staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-S100-B Antibody

Immunofluorescent analysis of S100-B staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-S100-B Antibody [orb705594]

    IH

    Human

    Mouse

    Monoclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Anti-S100-B Antibody [orb1474086]

    WB

    Mouse

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • Anti-S100-B Antibody [orb1421471]

    IHC

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 50 μl