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CD8b Antibody

SKU: orb699861

Description

Mouse monoclonal antibody to CD8b

Research Area

Epigenetics, Immunology

Images & Validation

Tested ApplicationsFA, FC, IHC-Fr, IP, WB
ReactivityRat
Application Notes
Flow cytometry: Recommended dilution: 1-4 µg/ml.Functional application: In vitro stimulation, in vivo depletion.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.341
ImmunogenCD8 positive Wistar rat splenic T cell hybridomas
TargetCD8b
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Ly-3, Ly-C, Lyt-3

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CD8b Antibody

Separation of murine CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 μg/ml, GAM APC).

CD8b Antibody

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 μg/ml, GAM APC).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
View Protocol

CD8b Antibody (orb699861)

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Available Sizes

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0.1 mg
$ 280.00
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