Anti-RPL27 Antibody

• Catalog Number: orb667988
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RPL27
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human RPL27. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RPL27
• Entrez: 6155, 19942, 64306
• UniProt ID: P61358, P61354, P61353
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-60S ribosomal protein L27 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RPL27 expression in A431 (A), HEK293T (B), mouse liver (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)

Immunofluorescent analysis of RPL27 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.