RNF5 Antibody

• Catalog Number: orb382011
• Category: Antibodies
• Description: The RNF5 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human RNF5 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human RNF5
• UniProt ID: Q5M807, Q99942, O35445
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5;
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 54197, 6048, 407784

Western blot analysis of RNF5 expression in MCF7 (A), Jurkat (B), mouse liver (C), mouse kidney (D), rat brain (E) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 21 kD)

Immunohistochemical analysis of RNF5 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.