RB1 (Phospho-T821) Antibody

• Catalog Number: orb665490
• Category: Antibodies
• Description: The RB1 (Phospho-T821) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1 protein. The exact sequence is proprietary.
• UniProt ID: P06400, P33568, P13405
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200, IF: 1-100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Retinoblastoma-associated protein; p105-Rb; pRb; R
• Research Area: Cancer, Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 19645, 5925, 24708
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RB1 (Phospho-T821) expression in HuT78 (A), Myla2059 (B), MCF7 (C) whole cell lysates. (Predicted band size: 106 kD; Observed band size: 120 kD)

Immunohistochemical analysis of RB1 (Phospho-T821) staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of RB1 (Phospho-T821) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.