Phospho-Rb (Thr821) Rabbit Polyclonal Antibody

• Catalog Number: orb6862
• Category: Antibodies
• Description: Phospho-Rb (Thr821) Rabbit Polyclonal Antibody
• Target: RB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Thr821 LP(p-T)PT
• UniProt ID: P06400
• RRID: AB_10932102
• MW: 102 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Rb(phospho T821); p-Rb(phospho T821); Rb(phospho-T
• Note: For research use only

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-Rb (Thr821)) polyclonal Antibody, Unconjugated (orb6862) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Rat Thymus tissue lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human Jurkat cell lysates, Primary: Anti-Phospho-Rb (Thr821) (orb6862) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 102 kDa, Observed band size: 125 kDa.