RAD50 Antibody

SKU: orb214962

Description

The RAD50 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, DNA Damage, Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Reactivity: Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD50. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

DNA repair protein RAD50; hRAD50

Western blot analysis of RAD50 expression in HeLa (A), K562 (B), Jurkat (C), MCF7 (D), Ramos (E), SW480 (F) whole cell lysates. (Predicted band size: 138; 153; 154 kD; Observed band size: 154 kD)

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P70388, Q92878, Q9JIL8

NCBI Gene ID

64012, 10111