RAD50 Rabbit Polyclonal Antibody

SKU: orb185928

Description

RAD50 Rabbit Polyclonal Antibody

Research Area

DNA Repair, DNA and RNA, DNA and RNA Research, DNA and RNA Researchh, Homelogous Recombination, Telomeres

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=0.2μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RAD50 (817-872/1312aa)
• Target: RAD50
• Molecular Weight: 154 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog(S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein.

Blank control: 293T cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-RAD50 antibody (orb185928), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-RAD50 (orb185928) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 154kD, Observed band size: 154kD.

Quick Database Links

Gene Symbol

RAD50

UniProt

Q92878