RAB7 RAB7A Rabbit Monoclonal Antibody

• Catalog Number: orb548029
• Category: Antibodies
• Description: Anti-RAB7 RAB7A Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human RAB7
• UniProt ID: P51149
• MW: 23 kDa
• Tested applications: FC, ICC, IF, IHC, WB
• Application notes: WB 1:1000-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: GHA-18
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ras-related protein Rab-7a; RAB7A; RAB7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAB7 using anti-RAB7 antibody (orb548029). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB7 antigen affinity purified monoclonal antibody (Catalog # orb548029) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for RAB7 at approximately 23 kDa. The expected band size for RAB7 is at 23 kDa.