RAB7A Recombinant Rabbit Monoclonal Antibody

SKU: orb1499334

Description

RAB7A Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: R2B4
• Immunogen: A synthesized peptide derived from human Rab7A (150-207/207aa)
• Target: RAB7A
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CMT2B; Member RAS oncogene family; RAB7A; Ras associated protein RAB7; Ras related protein Rab7; Ras related protein Rab7a; RAB7A_HUMAN.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: A431, 3: MCF-7, 4: NIH/3T3, 5: C6, Protein loading quantity: 20 ug, Exposure time: 60 s, Predicted MW: 23 kDa, Observed MW: 23 kDa.

Tissue: Human breast carcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499334.

Quick Database Links

Gene Symbol

RAB7A

UniProt

P51149