Anti-Pyridoxal Kinase Antibody

• Catalog Number: orb1474170
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pyridoxal Kinase
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human Pyridoxal Kinase. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/1000 - 1/2000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PDXK
• Entrez: 8566
• UniProt ID: O00764
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: C21orf124; C21orf97; PKH; PNK; Pyridoxal kinase; P
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Pyridoxal Kinase expression in 293 (A), MCF7 (B), U251 MG (C), human liver (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 36 kD)

Immunohistochemical analysis of Pyridoxal Kinase staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.