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PDXK Antibody (N-term)
Catalog Number: orb1925552
| Catalog Number | orb1925552 |
|---|---|
| Category | Antibodies |
| Description | Purified Mouse Monoclonal Antibody (Mab) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 1007CT2.1.1 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | IgG1,k |
| Dilution range | WB: 1:4000, IHC-P: 1:25 |
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 35102 Da |
| Target | This PDXK antibody is generated from a mouse immunized with a recombinant protein between 1-312 amino acids from human PDXK. |
| UniProt ID | O00764 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Pyridoxal kinase, 2.7.1.35, Pyridoxine kinase, PDX Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-PDXK Antibody (N-term) at 1:4000 dilution. Lane 1: 293 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: Human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining PDXK in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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