Anti-PRAS40 Antibody

• Catalog Number: orb304683
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRAS40
• Target: AKT1S1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRAS40. The exact sequence is proprietary.
• UniProt ID: Q96B36, Q9D1F4
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PRAS40 antibody, anti Proline-rich AKT1 subst
• Note: For research use only
• Entrez: 67605, 84335

Western blot analysis of PRAS40 expression in HepG2 (A), EC9706 (B), rat kidney (C) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 40 kD)

Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.