Phospho-AKT1S1 (Thr246) Rabbit Polyclonal Antibody

SKU: orb6793

Description

Phospho-AKT1S1 (Thr246) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PRAS40 around the phosphorylation site of Thr246 LN(p-T)SD
• Target: AKT1S1
• Molecular Weight: 28 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PRAS40(phospho Thr246); PRAS40(phospho T246); AKT1 substrate 1(proline rich); PRAS40; AKT1 substrate 1; AKT1-S1; AKT1S-1; AKT1S 1; AKT1 S1; Lobe; PRAS 40; PRAS-40; Proline rich akt substrate; 40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKTS1_HUMAN; MGC2865; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Phospho-AKT1S1 (Thr246)) Polyclonal Antibody, Unconjugated (orb6793) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

AKT1S1

UniProt

Q96B36

References

Chao Wang 1, Duyin Jiang Exogenous PRAS40 reduces KLF4 expression and alleviates hypertrophic scar fibrosis and collagen deposition through inhibiting mTORC1 Burns ., (2024)

PubMed: 38369439

Applications

IF

Reactivity

Rat