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Anti-PKC iota/zeta Antibody

Catalog Number: orb338857

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb338857
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKCI
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rabbit, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC iota/zeta. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPRKCI; PRKCZ
Entrez5590, 18759, 18762, 84006, 5584, 25522
UniProt IDQ62074, Q02956, P41743, Q05513, F1M7Y5, P09217
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti DXS1179E antibody, anti Protein kinase C iota
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PKC iota/zeta Antibody

Western blot analysis of PKC iota/zeta expression in MCF7 (A), NIH3T3 (B), mouse kidney (C), rat kidney (D) whole cell lysates. (Predicted band size: 68; 67 kD; Observed band size: 78 kD)

Anti-PKC iota/zeta Antibody

Immunohistochemical analysis of PKC iota/zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PKC iota/zeta Antibody

Immunofluorescent analysis of PKC iota/zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Anti-PKC iota/zeta (Phospho-T412/410) Antibody [orb393138]

    IF,  IH,  WB

    Human, Mouse, Rabbit, Rat, Zebrafish

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 200 μl, 100 μl
  • Anti-PKC iota/zeta (pT412/410) Antibody [orb737126]

    IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • PKC iota/zeta peptide [orb339251]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • PRKCI (Phospho-T412/410) Blocking Peptide [orb392840]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg