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Anti-PKC iota/zeta (Phospho-T412/410) Antibody

Catalog Number: orb393138

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb393138
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKCI (Phospho-T412/410)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rabbit, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T412/410 of human PKC iota/zeta protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPRKCI; PRKCZ
Entrez5590, 18759, 18762, 84006, 5584, 25522
UniProt IDQ62074, Q02956, P41743, Q05513, F1M7Y5, P09217
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesDXS1179E; Protein kinase C iota type; Atypical pro
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PKC iota/zeta (Phospho-T412/410) Antibody

Western blot analysis of PKC iota/zeta (Phospho-T412/410) expression in Hela (A), mouse lung (B) whole cell lysates. (Predicted band size: 68; 67 kD; Observed band size: 78 kD)

Anti-PKC iota/zeta (Phospho-T412/410) Antibody

Immunohistochemical analysis of PKC iota/zeta (Phospho-T412/410) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PKC iota/zeta (Phospho-T412/410) Antibody

Immunofluorescent analysis of PKC iota/zeta (Phospho-T412/410) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.