Anti-PI3K p85 alpha Antibody

• Catalog Number: orb334783
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PI3K p85 alpha
• Target: PIK3R1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant protein corresponding to human PI3K p85 alpha.
• UniProt ID: P27986
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Phosphatidylinositol 3-kinase regulatory subu
• Note: For research use only
• Entrez: 5295

Western blot analysis of PI3K p85 alpha expression in HepG2 (A), 293T (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 85 kD)

Immunohistochemical analysis of PI3K p85 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.