Phospho-EIF2S1/EIF2S1 Rabbit Monoclonal Antibody

SKU: orb548654

Description

Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

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Tested Applications	FC, ICC, IF, IHC, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 FC 1:40
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	IO-5
Immunogen	A synthesized peptide derived from human Phospho-EIF2S1 (S51)
Target	Eukaryotic translation initiation factor 2 subunit 1
Molecular Weight	36 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2S1; EIF2A

Quality Guarantee

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Phospho-EIF2S1/EIF2S1 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-EIF2S1 (S51) Antibody.

Western blot analysis of Phospho-EIF2S1 (Ser51) expression in (1) HeLa cell lysates treated with Calyculin A; (2) Untreated HeLa cell lysates.

ICC staining Phospho-eIF-2a (S51) in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.