Phospho-EGFR/EGFR Rabbit Monoclonal Antibody

SKU: orb548563

Description

Anti-Phospho-EGFR (Y1092) Rabbit Monoclonal Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Research Area

Cancer Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IP, WB
• Dilution Range: WB 1:500-2000 ICC/IF 1:50-200 IP 1:20 FC 1:20
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: HOF-5
• Immunogen: A synthesized peptide derived from human EGFR Full-length sequence 1210aa around the phosphorylation site of Tyrosine 1092
• Target: Epidermal growth factor receptor
• Molecular Weight: 185 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epidermal growth factor receptor; 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1

Western blot analysis of Phospho-EGFR (Y1068) expression in (1) A431 cell lysate; (2) A431 cell treated with EGF lysate.

Quick Database Links

Gene Symbol

Epidermal growth factor receptor

UniProt

P00533