Phospho-EGFR (S695) Rabbit Monoclonal Antibody

• Catalog Number: orb548564
• Category: Antibodies
• Description: Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody. Tested in WB, ICC/IF applications. This antibody reacts with Human.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human EGFR
• UniProt ID: P00533
• MW: 185 kDa
• Tested applications: ICC, IF, WB
• Application notes: WB 1:500-2000ICC/IF 1:50-200
• Antibody Type: Primary Antibody
• Clone Number: GCC-5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Epidermal growth factor receptor; 2.7.10.1; Proto-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phospho-EGFR (S695) expression in A431 cell lysate treated with EGF (orb548564). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # orb548564) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for EGFR.