Peripherin Antibody

SKU: orb319633

Description

Our Anti-Peripherin primary antibody from Biorbyt is chicken polyclonal. It detects bovine, human, mouse, and rat Peripherin and is total IgY fraction.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:500-1:2000, ICC: 1:500-1:5000
• Reactivity: Bovine, Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine, Feline, Goat, Guinea pig, Hamster, Primate, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Recombinant rat peripherin expressed in and purified from E. coli.
• Target: Peripherin
• Molecular Weight: 57
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 21.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEF 4 antibody, NEF4 antibody, Neurofilament 4 (57kD) antibody, Neurofilament 4 antibody, Perf antibody, PERI_HUMAN antibody, Peripherin antibody, PRPH 1 antibody, prph antibody, PRPH1 antibody

Immunoflourescence of cultured rat neurons and glia showing peripherin (Cat. orb319633, green, 1:500) and alpha-internexin (red). The blue stain is nuclear DNA. Cells expressing both peripherin and alpha-internexin appear yellow.

Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (Cat. orb319633, green, 1:5000) and anti-vimentin (red). The blue is DAPI staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.

Quick Database Links

Gene Symbol

Peripherin

UniProt

P21807