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Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 0.736111111, IHC: 0.180555556, ICC: 1:200-1:500 |
| Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 7C5 |
| Immunogen | Recombinant rat peripherin expressed in and purified from E. coli. |
| Target | Peripherin |
| Molecular Weight | 57 |
| Purification | Concentrated tissue culture supernatant |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Concentrated tissue culture supernatant + 10 mM NaN3 |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NEF 4 antibody, NEF4 antibody, Neurofilament 4 (57kD) antibody, Neurofilament 4 antibody, Perf antibody, PERI_HUMAN antibody, Peripherin antibody, PRPH 1 antibody, prph antibody, PRPH1 antibody
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Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (Cat. orb319632, red, 1:500) and anti-vimentin (Cat. orb319707, green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunostaining of cultured newborn rat neurons and glia showing peripherin (Cat. orb319632, green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~57 kDa peripherin protein.
Quick Database Links
Gene Symbol
Peripherin
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Peripherin Antibody (orb319632)
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