Anti-Perforin FITC

• Catalog Number: orb1562575
• Category: Antibodies
• Description: Perforin Antibody (FITC)
• Target: Perforin
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: FITC
• Reactivity: Bovine, Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: purified granules from human YT lymphoma cell line
• UniProt ID: P14222
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
• Clone Number: dG9
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: PRF1, P1, PFP, HPLH2
• Note: For research use only
• Entrez: 5551

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human Perforin (dG9) FITC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human Perforin positive CD56 positive lymphocytes (red-filled) from Perforin negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Perforin (dG9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).