PEDF Antibody

SKU: orb315701

Description

The PEDF Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PEDF. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1-500:1000
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PEDF. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

PEDF; Pigment epithelium-derived factor; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

Western blot analysis of PEDF expression in HepG2 (A), A2780 (B), PC3 (C), 3T3L1 (D), H9C2 (E) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 43 kD)

Immunohistochemical analysis of PEDF staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P36955, P97298

NCBI Gene ID

20317, 5176