PEDF rabbit pAb Antibody

SKU: orb769440

Description

Rabbit polyclonal antibody to PEDF

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PEDF. AA range:258-307
• Molecular Weight: 46
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-SERPINF1 antibody, anti-PEDF antibody, anti-PIG35 antibody, anti-Pigment epithelium-derived factor antibody, anti-PEDF antibody, anti-Cell proliferation-inducing gene 35 protein antibody, anti-EPC-1 antibody, anti-Serpin F1 antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunohistochemistry analysis of PEDF antibody in paraffin-embedded human prostate carcinoma tissue.

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night.

Quick Database Links

UniProt

P36955