Anti-PD-1H [MH5A]

SKU: orb758788

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: Blocking, ELISA, IHC
• Reactivity: Mouse

Key Properties

• Host: Hamster
• Clonality: Monoclonal
• Isotype: Hamster IgG
• Clone No.: MH5A
• Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
• Target: PD-1H
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor

ELISA of anti-VISTA antibody on VISTA-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (orb758788; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using orb758788. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A (orb758788) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758788, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

PD-1H

UniProt

Q9D659