Anti-PD-1H [MH5A]

SKU: orb758787

Description

Hamster monoclonal antibody to PD-1H

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: Blocking, ELISA, IHC
• Reactivity: Mouse

Key Properties

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MH5A
• Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
• Target: PD-1H
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor

ELISA of anti-VISTA antibody on VISTA-Fc fusion protein. Binding curve of the rabbit chimeric version of the anti-VISTA antibody MH5A (orb758788; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using orb758788. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A (orb758788) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758788, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

PD-1H

UniProt

Q9D659