Anti-PCSK1 Antibody

• Catalog Number: orb1879163
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PCSK1
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human PCSK1. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/30)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 5122, 100911216, 18548, 25204
• UniProt ID: P63239, P29120, P28840
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: NEC1; Neuroendocrine convertase 1; NEC 1; Prohormo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PCSK1 expression in Hela (A), A549 (B), PC12 (C), mouse brain (D), NCIH460 (E) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 96 kD)

Flow cytometric analysis of A549 cells using Anti-PCSK1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.