Anti-p38 (Phospho-T180) Antibody

• Catalog Number: orb216074
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p38 (Phospho-T180)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T180 of human p38 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAPK14
• Entrez: 1432, 26416
• UniProt ID: Q16539, P47811, P70618
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CSBP antibody, anti CSBP1 antibody, anti CSBP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p38 (Phospho-T180) expression in zebrafish (A) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 43 kD)

Immunofluorescent analysis of p38 (Phospho-T180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of p38 (Phospho-T180) expression in mouse liver (A), rat liver (B) whole cell lysates.

Direct ELISA antibody dose-response curve using Anti-p38 (Phospho-T180) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.