Anti-p16 INK4a Antibody

• Catalog Number: orb213717
• Category: Antibodies
• Description: Rabbit polyclonal antibody against CDKN2A
• Target: CDKN2A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p16 INK4a. The exact sequence is proprietary.
• UniProt ID: P42771, Q9R0Z3, P51480
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-ARF antibody, anti-CCM2 antibody, anti-CDK4 i
• Note: For research use only
• Entrez: 25163, 1029, 12578

Western blot analysis of p16 INK4a expression in HEK293T (A), HeLa (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 20 kD )

Immunohistochemical analysis of p16 INK4a staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.