Anti-NUP50 Antibody

• Catalog Number: orb667537
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NUP50
• Target: NUP50
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human NUP50. The exact sequence is proprietary.
• UniProt ID: Q9UKX7, O08587, Q9JIH2
• Tested applications: IH, WB
• Dilution range: WB: 1-500-2000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-NPAP60L antibody, anti-Nuclear pore complex p
• Note: For research use only
• Entrez: 25497, 10762, 18141

Western blot analysis of NUP50 expression in HeLa (A), mouse thymus (B) whole cell lysates. (Predicted band size: 46; 50 kD; Observed band size: 50 kD)

Immunohistochemical analysis of NUP50 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.