NUP214 Antibody

• Catalog Number: orb667534
• Category: Antibodies
• Description: The NUP214 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human NUP214. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human NUP214. The exact sequence is proprietary.
• UniProt ID: Q80U93, P35658
• Tested applications: IF, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CAIN; CAN; KIAA0023; Nuclear pore complex protein
• Research Area: Cancer, Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 8021, 227720

Western blot analysis of NUP214 expression in HEK293T (A), mouse brain (B) whole cell lysates. (Predicted band size: 212; 213; 215 kD; Observed band size: 251 kD)

Immunofluorescent analysis of NUP214 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.