NUP214 Rabbit Polyclonal Antibody

• Catalog Number: orb312369
• Category: Antibodies
• Description: NUP214 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC
• Predicted Reactivity: Mouse, Primate
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NUP214 (2-100/2080aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 213 kDa
• Target: NUP214
• UniProt ID: P35658
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 214 kDa nucleoporin; CAIN; CAN; CAN protein putati
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (NUP214) Polyclonal Antibody, Unconjugated (orb312369) 1:200, 90 minutes at 37°C, followed by a conjugated secondary antibody (orb868805) at 37°C for 90 minutes, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.