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Anti-NRG1 Antibody

Catalog Number: orb215474

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb215474
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NRG1
TargetNRG1
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NRG1. The exact sequence is proprietary.
UniProt IDP43322, Q02297
Tested applicationsIF, WB
Dilution rangeWB: 1-500-1-1000
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti GGF antibody, anti HGL antibody, anti HRGA an
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NoteFor research use only
Entrez3084, 112400
Anti-NRG1 Antibody

Western blot analysis of NRG1 expression in mouse liver (A), mouse spleen (B), rat liver (C), rat spleen (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70; 45 kD)

Anti-NRG1 Antibody

Immunofluorescent analysis of NRG1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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