NPB Antibody

SKU: orb667485

Description

The NPB Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human NPB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human NPB. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPL7; PPNPB; Neuropeptide B; Preproprotein L7; hPPL7

Western blot analysis of NPB expression in SW480 (A), mouse brain (B) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 13 kD)

Immunofluorescent analysis of NPB staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q8NG41, Q8K4P2, Q8K4P1

NCBI Gene ID

259222, 208990, 256933