NMDAR2D Rabbit Polyclonal Antibody

SKU: orb393066

Description

The NMDAR2D Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NMDAR2D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500:1000
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NMDAR2D. The exact sequence is proprietary.
• Target: GRIN2D
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GluN2D; NMDAR2D; Glutamate receptor ionotropic, NMDA 2D; GluN2D; EB11; Glutamate [NMDA] receptor subunit epsilon-4; N-Methyl-D-aspartate receptor subtype 2D; NMDAR2D; NR2D

Western blot analysis of NMDAR2D expression in A549 (A) whole cell lysates. (Predicted band size: 143 kD; Observed band size: 165 kD)

Immunofluorescent analysis of NMDAR2D staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

GRIN2D

UniProt

Q62645, Q03391, O15399

NCBI Gene ID

14814, 2906, 24412