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Item 1 of 3
Anti-NLRP1 Antibody
Catalog Number: orb1289704
| Catalog Number | orb1289704 |
|---|---|
| Category | Antibodies |
| Description | Rabbit Polyclonal antibody to NLRP1 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IH, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant protein corresponding to human NLRP1. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | NLRP1 |
| Entrez | 22861 |
| UniProt ID | Q9C000 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | CARD7; DEFCAP; KIAA0926; NAC; NALP1; NACHT LRR and Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of NLRP1 expression in SHSY5Y (A) whole cell lysates. (Predicted band size: 46; 83; 154-165 kD; Observed band size: 160 kD)
Immunohistochemical analysis of NLRP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NLRP1 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
