Neurofilament NF-M Antibody

SKU: orb319588

Description

Our Anti-Neurofilament NF-M primary antibody from Biorbyt is mouse monoclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-M and is Protein G purified.

Research Area

Cell Biology, Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:500-1:5000, ICC: 1:100-1:500
• Reactivity: Bovine, Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3H11
• Immunogen: Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.
• Target: Neurofilament NF-M
• Molecular Weight: 145
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 2 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody

Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (Cat. orb319588, 1:5000, red) and anti-Neurofilament-H (Cat. orb319585, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.

Immunostaining of cultured rat neurons showing labeling of NF-M (Cat. orb319588, green, 1:100) in mature neurons.

Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

Quick Database Links

Gene Symbol

Neurofilament NF-M

UniProt

P12839