NF-H Antibody (phospho)

SKU: orb749716

Description

This mAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/10^6 cells,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the NF-H antibody (phospho) should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NE14
• Immunogen: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with NF-H antibody (NE14).

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with NF-H antibody (NE14).

SDS-PAGE Analysis of Purified, BSA-Free NF-H Antibody (clone NE14). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human brain lysate with NF-H antibody (clone NE14).

Flow cytometry testing of permeabilized human HEK293 cells with NF-H antibody (clone NE14); Red = isotype control, Blue = NF-H antibody.

Quick Database Links

UniProt

P12036