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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 3.513888889, IHC: 1:500-1:1000, ICC: 1:500-1:1000 |
| Reactivity | Gallus, Mammal |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | NAP4 |
| Immunogen | Purified bovine NF-H |
| Target | Neurofilament NF-H |
| Molecular Weight | 200 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 10 mM NaN3. |
| Concentration | 2 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunostaining of human cerebellar cortex showing labeling of NF-H (Cat. orb319584, DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
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Protocol Information
Neurofilament NF-H Antibody (orb319584)
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