Anti-NEK1 Antibody

• Catalog Number: orb1879492
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NEK1
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human NEK1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 4750
• UniProt ID: Q96PY6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA1901; Serine/threonine-protein kinase Nek1; Ne
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NEK1 expression in human fetal kidney (A) whole cell lysates. (Predicted band size: 142 kD; Observed band size: 143 kD)

Immunohistochemical analysis of NEK1 staining in human hepatocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.